What drives pediatric Burkitt lymphoma? Timing events in the evolution of cancer using single‐cell whole genome sequencing

Introduction: Burkitt lymphoma (BL) is a rare but highly aggressive B-cell non-Hodgkin lymphoma. BL exhibits characteristic immunophenotype that positive for pan-B cell markers and CD10. The genetic hallmark of the translocation MYC oncogene under regulation an immunoglobulin (IG) heavy or light chain enhancer, resulting in protein overexpression. Notably, by itself not sufficient oncogenesis variety cancer genes are recurrently mutated. can be divided epidemiologically also, more recently, genetically based on driver mutations including DGG-BL (DDX3X, GNA13, GNAI2), IC-BL (ID3 CCND3), Q53-BL (quiet TP53). Despite high survival rates, pediatric patients suffer from long-term side effects relapses usually fatal. In order to develop targeted less toxic therapies, better understanding needed ethology disease. Therefore, our aim characterise cell-of-origin (COO) dissect life history subtypes, pinpointing when during tumorigenesis somatic play role identifying rate-limiting steps malignant transformation. Method: We collected single-cell suspensions patient samples, lymph node biopsies, bone marrow aspirates, as well ascites pleural fluid. To perform flow cytometry, single cells were stained with DAPI panel antibodies: CD10/CD45/CD19 CD10/CD3/CD20. Live B separated DAPI-CD45+ CD19+ DAPI-CD3-CD20+ phenotype. Subsequently, CD10+ CD10- sorted 96-well plates (Figure 1). confirm presence absence IG::MYC PCR was perfomed using patient-specific primers flank locus. Whole genome amplification carried out DNA Primary Template-directed Amplification (PTA) technique subsequently whole sequenced. Results: From sample 4-year-old female we found 1% immune cells, while 17% negative CD10 marker 2A). same patient, 87% 9% 2B). confirmed 2/6 0/6 marrow-derived 3). Keywords: Genomics, Epigenomics, Other -Omics, Non-Hodgkin (Pediatric, Adolescent, Young Adult), Tumor Biology Heterogeneity No conflicts interests pertinent abstract.